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KMID : 0903520090520030283
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 3 p.283 ~ p.289
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Effect of Non-animal-Derived Nitrogen Sources on the Production of Hyaluronic Acid by Streptococcus sp. KL0188
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Lee Gil-Yong
Ha Suk-Jin
Jung Jong-Hyun
Seo Dong-Ho
Park Jong-Yul
Kim Su-Rin
Park Nam-Woo
Kweon Dong-Keon
Park Sang-Hoo
Park Cheon-Seok
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Abstract
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Hyaluronic acid (HA) is a linear high-molecular-weight polysaccharide with useful biomedical applications. Streptococcus zooepidemicus, a typical HA-producing bacterium, requires an animal-derived nitrogen source such as tryptone, peptone or sheep blood as a nutrient. Sixteen nonanimal-derived (NAD) nitrogen sources were tested as a replacement for the expensive animal-derived nitrogen sources, which may have safety issues. Among the sixteen tested NAD nitrogen sources, a yeast-derived nitrogen source (YE 0251) showed the highest HA productivity, which was equivalent to the control HA production medium containing tryptone in a 5-L jar and in 3,000-L industrial fermentations. In the 3,000-L fermentation, YE 0251 increased cell mass (dry cell weight) and HA production by 11% and 8%, respectively, compared with the control HA production medium. The final specific volumetric productivity (0.41 g/L · h) was improved by about 70% after reducing the fermentation time from 20 h to 12 h, compared to the conventional production medium.
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KEYWORD
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hyaluronic acid, Streptococcus zooepidemicus, tryptone
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